Transcriptome Analysis for Fraxinus mandshurica Rupr. Seedlings from Different Carbon Sequestration Provenances in Response to Nitrogen Deficiency

To explore the molecular regulatory mechanism of high-carbon (C) sequestration Fraxinus mandshurica Rupr. (F. mandshurica) provenance and expression profile F. during nitrogen (N) starvation, foliage roots annual Wuchang (WC) seedlings with greater C amount Hailin (HL) smaller amount, which were grown in N-deficient nutrition complete N, used for RNA-seq physiological determination, respectively. One thousand fifty-seven differentially expressed genes (DEGs) between WC HL 8173 DEGs related to N deficiency identified, The root responded more strongly than foliar. target that mainly (transcription factors, hormones protein kinases), their response patterns upregulated. growth concentration both reduced by deficiency, might result from decrease leaf Nitrate reductase (NR) glutamine synthetase (GS) enzyme activity ABA content, although root-to-shoot ratio; lateral number; lignin content; endogenous content (GA, IAA ZR); GS glutamate transcriptional level most increased. capacity was HL, higher enzymes levels metabolic (terpenes, carbohydrates, lipid energy). However, advantage significantly due compared HL.